Science Links Japan | Identification and Isolation of Foot-and-Mouth Disease Virus from Primary Suspect Cases in Korea in 2000.

Identification and Isolation of Foot-and-Mouth Disease Virus from Primary Suspect Cases in Korea in 2000.

Accession number;03A0101991

Title;Identification and Isolation of Foot-and-Mouth Disease Virus from Primary Suspect Cases in Korea in 2000.

Author; SHIN J-H (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) SOHN H-J (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) KWON B-J (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) CHOI C-U (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) KIM J-H (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) PARK J-H (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) KIM J-Y (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) CHOI S-H (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor) KIM O-K (National Veterinary Res. And Quarantine Serv., Gyeonggi, Kor)

Journal Title;J Vet Med Sci

Journal Code:F0905A

ISSN:0916-7250

VOL.65;NO.1;PAGE.1-7(2003)

Figure&Table&Reference;FIG.3, TBL.2, REF.24

Pub. Country;Japan

Language;English

Abstract;The Republic of Korea had been free from foot and mouth disease (FMD) since 1934, until a recent outbreak in 2000. From March to April 2000, a total of 15 FMD outbreaks due to the serotype O virus were recorded. Coincidental outbreaks of FMD in cattle or pigs by the serotype O virus were reported in the region, including Taiwan, China, Japan, Russia and Mongolia. In this report, the results of emergency investigations of FMD cases on a dairy farm located approximately 5-km from the demilitarized zone in Korea are described. The causative agent of the disease was identified as the FMD virus O by reverse transcription-polymerase chain reaction (RT-PCR) assays using primers derived from the 3D polymerase, internal ribosome entry site (IRES), 1D/2B regions, enzyme-linked immunosorbent assay (ELISA) for antigen detection and typing. Sequence data of the partial 1D/2B region obtained from vesicular fluid showed close similarity (98% sequence identity) to the Kinmen isolate of the FMD virus O in Taiwan. The causative virus was isolated using black goat fetal lung cells following propagation in unweaned mice. (author abst.)

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