Science Links Japan | A Study on the Number of Recovered Spermatozoa in the Uterine Horns and Oviducts of Gilts, after Fractionated or Non-Fractionated Insemination.

A Study on the Number of Recovered Spermatozoa in the Uterine Horns and Oviducts of Gilts, after Fractionated or Non-Fractionated Insemination.

Accession number;03A0102000

Title;A Study on the Number of Recovered Spermatozoa in the Uterine Horns and Oviducts of Gilts, after Fractionated or Non-Fractionated Insemination.

Author; KUNAVONGKRIT A (Chulalongkorn Univ., Bangkok, Tha) SANG-GASANEE K (Chulalongkorn Univ., Bangkok, Tha) PHUMRATANAPRAPIN C (Chulalongkorn Univ., Bangkok, Tha) TANTASUPARUK W (Chulalongkorn Univ., Bangkok, Tha) EINARSSON S (Swedish Univ. Agricultural Sci. Uppsala, Swe)

Journal Title;J Vet Med Sci

Journal Code:F0905A

ISSN:0916-7250

VOL.65;NO.1;PAGE.63-67(2003)

Figure&Table&Reference;FIG.1, TBL.4, REF.13

Pub. Country;Japan

Language;English

Abstract;The objective of this study was to compare the number of recovered spermatozoa, in different parts of the uterine horn and oviduct in guts, after insemination with fractionated (experiment) and non-fractionated (control) liquid stored semen. The number of spermatozoa and volume of backflow was also investigated. Twenty three cross-bred gilts were used in the study. They were divided into 2 groups, a control group (non-fractionated liquid stored semen, n=10) which were inseminated with 100ml of liquid stored semen containing 3,000 million spermatozoa per dose and an experimental group (fractionated liquid stored semen, n=10) which were inseminated with 50ml of liquid stored semen, with 3,000 million spermatozoa per dose and followed by another 50ml of semen dilutor (Beltsville Thawing Solution, BTS). Thereafter, backflow semen was collected and measured every 15min for a period of 1 hr. Three or 12 hr after insemination, 5 gilts from each group had the uterus, the horn of the uterus, the oviducts and the ovaries removed under general anaesthesia. The horn of uterus and the oviducts were seperated by ligation into 6 segments. All 6 segments were flushed with BTS to collect all spermatozoa within the segment. Recovered spermatozoa were counted, using a haemocytometer and the volume recorded. It was seen that the percentage of spermatozoa in the backflow semen in the experimental group was less than in the control group. The difference was not significant in the gilts that were operated on 3 hr after insemination, the mean number of spermatozoa in the uterine horn and the utero-tubular junction (UTJ) was more in the experimental than in the control group, but less in the isthmus and the ampulla of the oviduct. The gilts which were operated on 12 hr after insemination, had relativity more ovulating gilts in the control group than in the experimental group (3 of 4 gilts compare to 3 of 5 gilts).... (author abst.)

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