Protective Effects of Oral Microencapsulated Mycoplasma hyopneumoniae Vaccine Prepared by Co-Spray Drying Method.
|
Accession number;03A0102001
|
| Title;Protective Effects of Oral Microencapsulated Mycoplasma hyopneumoniae Vaccine Prepared by Co-Spray Drying Method. |
| Author;
LIN J H
(National Taiwan Univ., Taipei, Twn)
WENG C N
(Animal Technol. Inst. Taiwan, Miaoli, Twn)
LIAO C W
(Animal Technol. Inst. Taiwan, Miaoli, Twn)
YEH K S
(Animal Technol. Inst. Taiwan, Miaoli, Twn)
PAN M J
(National Taiwan Univ., Taipei, Twn)
|
Journal Title;J Vet Med Sci
|
Journal Code:F0905A
|
ISSN:0916-7250
|
|
VOL.65;NO.1;PAGE.69-74(2003)
|
| Figure&Table&Reference;FIG.2, TBL.3, REF.27 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The efficacy of Mycoplasma hyopneumoniae oral vaccine was investigated in microsphere dosage form. A co-spray drying process was used to apply an encapsulating material, Eudragit L30 D-55, to microspheres containing Mycoplasma hyopneumoniae antigens. The microspheres were generally effective (>93%) with protein release at pH 7.4, but almost none were released at pH 1.2, for 3 hr in an in vitro dissolution test. An SPF-swine model was used to evaluate the effectiveness of the microspheres as an oral vaccine, and the related immune responses. The serum's systemic IgG against M hyopneumoniae was evoked by ELISA analysis, after a 2nd immunization of all pigs. The vaccinated groups' mean lesion score was significantly lower after the Mycoplasma hyopneumoniae challenge than that of the nonvaccinated/challenged groups (P<0.05). This study strongly suggests that the oral microspheres vaccine prepared by a co-spray drying method can provide effective protection against M. hyopneumoniae infection in pigs. (author abst.) |
|
|
|
Related Articles;
|
|
|
