Characterization of the Testis in Congenitally Ubiquitin Carboxy-Terminal Hydrolase-1(Uch-L1) Defective(gad) Mice.
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Accession number;03A0132757
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| Title;Characterization of the Testis in Congenitally Ubiquitin Carboxy-Terminal Hydrolase-1(Uch-L1) Defective(gad) Mice. |
| Author;
KWON J
(Univ. Tokyo, Tokyo, Jpn)
KIKUCHI T
(National Center Of Neurology And Psychiatry, Tokyo, Jpn)
SETSUIE R
(National Center Of Neurology And Psychiatry, Tokyo, Jpn)
ISHII Y
(Univ. Tokyo, Tokyo, Jpn)
KYUWA S
(Univ. Tokyo, Tokyo, Jpn)
YOSHIKAWA Y
(Univ. Tokyo, Tokyo, Jpn)
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Journal Title;Exp Anim
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Journal Code:Z0755A
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ISSN:1341-1357
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VOL.52;NO.1;PAGE.1-9(2003)
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| Figure&Table&Reference;FIG.6, TBL.3, REF.23 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The gracile axonal dystrophy(gad) mice are known to have a deletion within the gene encoding ubiquitin carboxy-terminal hydrolase-1(Uch-L1) and show hereditary sensory deterioration and motor paresis. Expression of Uch-L1 is reported to be almost limited to the nervous system and testis. To understand whether Uch-L1, one of the major ubiquitin carboxy-terminal hydrolase(UCH) isozymes in the testis, affects spermatogenesis and other UCH isozymes(Uch-L3, L4 and L5) expression in the testis, we compared the testis between gad, hetero and wild type mice by histological, immunohistochemical analyses and RT-PCR. Histological analysis in 25-week-old gad mice showed shrinking of seminiferous tubules, decreasing total number of cells and enlargement of remaining cells in seminiferous tubules. By immunohistochemistry, a significant decrease(p<0.05) in the number of proliferating cell nuclear antigen(PCNA) positive cells was observed. Expression of other UCH isozyme mRNAs was not apparently affected by Uch-L1 deficiency in 25-week-old gad mice. This study is the first report on the testis of gad mutant mouse. (author abst.) |
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