Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli.
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Accession number;03A0132760
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| Title;Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli. |
| Author;
TAKAKURA A
(Central Inst. Experimental Animals, Kawasaki, Jpn)
GOTO K
(Central Inst. Experimental Animals, Kawasaki, Jpn)
ITOH T
(Central Inst. Experimental Animals, Kawasaki, Jpn)
YOSHIMATSU K
(Hokkaido Univ., Sapporo, Jpn)
TAKASHIMA I
(Hokkaido Univ., Sapporo, Jpn)
ARIKAWA J
(Hokkaido Univ., Sapporo, Jpn)
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Journal Title;Exp Anim
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Journal Code:Z0755A
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ISSN:1341-1357
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VOL.52;NO.1;PAGE.25-30(2003)
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| Figure&Table&Reference;FIG.2, TBL.2, REF.18 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A recombinant nucleocapsid protein of Hantaan virus(HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay(rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay(IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR-11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples. (author abst.) |
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