Differences between BALB/c and C57BL/6 Mice in Mouse Hepatitis Virus Replication in Primary Hepatocyte Culture.
|
Accession number;03A0132769
|
| Title;Differences between BALB/c and C57BL/6 Mice in Mouse Hepatitis Virus Replication in Primary Hepatocyte Culture. |
| Author;
KYUWA S
(Univ. Tokyo, Tokyo, Jpn)
KAWAMURA S
(Univ. Tokyo, Tokyo, Jpn)
TAGAWA Y
(Univ. Tokyo, Tokyo, Jpn)
IWAKURA Y
(Univ. Tokyo, Tokyo, Jpn)
URANO T
(Kumamoto Univ., Kumamoto, Jpn)
YOSHIKAWA Y
(Univ. Tokyo, Tokyo, Jpn)
|
Journal Title;Exp Anim
|
Journal Code:Z0755A
|
ISSN:1341-1357
|
|
VOL.52;NO.1;PAGE.81-84(2003)
|
| Figure&Table&Reference;FIG.2, TBL.1, REF.15 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We previously showed that an intraperitoneal infection with mouse hepatitis virus(MHV) resulted in acute hepatic failure accompanying extremely elevated viral growth in the liver in interferon-.GAMMA.-deficient BALB/c(BALB-GKO), but not C57BL/6(B6-GKO) mice. To examine the basis of the strain difference against MHV infection in interferon-.GAMMA.-deficient mice, viral replication in primary hepatocyte cultures from BALB/c and B6 mice with or without the IFN-.GAMMA. gene was compared in vitro. The MHV replication in BALB/c hepatocytes with or without the IFN-.GAMMA. gene was significantly higher than that in B6 hepatocytes with or without the IFN-.GAMMA. gene, suggesting that there is a strain difference in MHV replication in hepatocytes. Since a significant difference in MHV replication in hepatocytes was not observed between wild type and IFN-.GAMMA.-deficient mice of the same genetic background, the phenomenon is thought to be independent of IFN-.GAMMA.. However, pretreatment of hepatocytes with recombinant mouse interferon-.GAMMA. inhibited MHV replication in a dose-dependent fashion. The results are discussed with respect to the pathology of MHV infection in mice with or without the IFN-.GAMMA. gene. (author abst.) |
|
|
|
Related Articles;
|
|
|
