Regulation of Corticotropin-Releasing Factor(CRF) Type-1 Receptor Gene Expression by CRF in the Hypothalamus.
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Accession number;03A0147505
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| Title;Regulation of Corticotropin-Releasing Factor(CRF) Type-1 Receptor Gene Expression by CRF in the Hypothalamus. |
| Author;
KONISHI S
(Nippon Medical School, Kawasaki, Jpn)
KASAGI Y
(Nippon Medical School, Kawasaki, Jpn)
KATSUMATA H
(Nippon Medical School, Kawasaki, Jpn)
MINAMI S
(Nippon Medical School, Kawasaki, Jpn)
IMAKI T
(Nippon Medical School, Kawasaki, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.50;NO.1;PAGE.21-36(2003)
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| Figure&Table&Reference;FIG.8, REF.48 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We reported previously that acute stress and intracerebroventricular(ICV) injection of corticotropin-releasing factor(CRF)increased neuronal activation and CRF type-1 receptor(CRFR-1) mRNA expression in the CRF-producing neurons of the parvocellular paraventricular nucleus(PVN) of the hypothalamus. In this study, to determine whether CRF can act directly on hypothalamic CRF neurons, thereby increasing CRFR-1 expression, microinjection of CRF into PVN neurons in vivo and primary cultures of dispersed rat fetal hypothalami in vitro were performed. Microinjection of 0.1.MU.g of CRF into the PVN significantly increased c-fos and CRFR-1 mRNA expression in the CRF-producing parvocellular PVN, 30min or 180min after injection, respectively. This effect was blocked by a CRF antagonist, .ALPHA.-helical CRF. CRF, when injected into the lateral ventricle at the same dose, increased neither CRFR-1 nor c-fos mRNA levels in the PVN. Primary culture of hypothalamic neurons revealed that CRFR-1 like immunoreactivity was located in CRF-containing neurons, and that the CRFR-1 mRNA level was significantly increased 4h after incubation with 10-8M CRF. These results demonstrate that CRF directly affects hypothalamic neurons to increase CRFR-1 mRNA expression, providing evidence of a direct role for CRF in the regulation of CRFR-1 expression of hypothalamic neurons. (author abst.) |
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