Novel Splicing Events of Untranslated First Exons in Human Estrogen Receptor Alpha(ER.ALPHA.) Gene.
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Accession number;03A0147514
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| Title;Novel Splicing Events of Untranslated First Exons in Human Estrogen Receptor Alpha(ER.ALPHA.) Gene. |
| Author;
OKUDA Y
(Univ. Yamanashi, Yamanashi, Jpn)
HIRATA S
(Univ. Yamanashi, Yamanashi, Jpn)
WATANABE N
(Univ. Yamanashi, Yamanashi, Jpn)
SHODA T
(Univ. Yamanashi, Yamanashi, Jpn)
KATO J
(Teikyo Univ. Inst. Global Education, Tokyo, Jpn)
HOSHI K
(Univ. Yamanashi, Yamanashi, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.50;NO.1;PAGE.97-104(2003)
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| Figure&Table&Reference;FIG.4, REF.18 |
| Pub. Country;Japan |
| Language;English |
| Abstract;In order to analyze the structures of the 5'-untranslated region of estrogen receptor alpha(ER.ALPHA.) mRNA in human uterine endometrium(Em), total RNA from Em was analyzed by 5'-rapid amplification of the cDNA ends method with antisense primer located on exon 1 of human ER.ALPHA. gene. Three isoforms of 5'-RACE clones were obtained: ER.ALPHA. mRNAs containing exon(A)(the upstream region of exon 1), exon C, and exons F-E2(we adopted the nomenclature of 5'-untranslated exons of the Gannon group). The results imply that the major isoforms of ER.ALPHA. mRNA expressed in Em are these three isoforms. Moreover, reverse transcription-polymerase chain reaction(RT-PCR) analysis was carried out on Em, ovary(Ov) and liver(Li) mRNAs to detect the novel isoforms of ER.ALPHA. mRNA in these tissues, using sense primers located on exons(A),B,C,F, and E1, and antisense primer located on exon 1 As a result, in addition to the previously reported ER.ALPHA. mRNA isoforms containing exons(A),B,C,F-E2 and E1-E2 on exon 1, we identified two novel isoform mRNAs in which exons F and E1 were directly spliced onto exon 1. Differential distributions of these isoforms of ER.ALPHA. mRNAs in Em, Ov and Li were demonstrated by RT-PCR-Southern blot analysis. These results, together with the previous reports by others, indicate that there are at least ten isoforms of ER.ALPHA. mRNA containing different 5'-untranslated regions, exons(A),B,C,D,T1-T2,T1,F-E2,F,E1-E2 and E1, expressed in human, and that these are involved in tissue specific expression of the gene. (author abst.) |
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