Analysis of hematopoietic differentiation using ES cell in vitro differentiation induction system.
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Accession number;03A0148973
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| Title;Analysis of hematopoietic differentiation using ES cell in vitro differentiation induction system. |
| Author;
KITAJIMA KENJI
(Res. Inst. for Microb. Dis., Osaka Univ.)
ERA TAKUMI
(Riken Hasseisaiseikagakusokense)
TANAKA MAKOTO
(Res. Inst. for Microb. Dis., Osaka Univ.)
NAKANO TOORU
(Res. Inst. for Microb. Dis., Osaka Univ.)
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Journal Title;Protein, Nucleic Acid and Enzyme
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Journal Code:F0325A
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ISSN:0039-9450
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VOL.48;NO.3;PAGE.223-231(2003)
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| Figure&Table&Reference;FIG.7, REF.34 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In the process of proceeding cell differentiation, cell-line specific or differentiation-step specific transcriptional control factors play main roles. Hematopoietic system, which produces up to 10 differentiated cells from a single undifferentiated cell, is no exception. Analysis of biological functions of transcription factors in differentiation of blood cells has been carried out using enforced gene expression such as using gene targeting, and retrovirus. These experiments, however, can analyze only an aspect of functions of transcription factors. By combining in vitro differentiation induction of blood cells from mouse embryonic stem cells(ES cells), and the system to be able to conditionally control expression of exogenous gene, gene expression is controlled time-relatedly to be expressed, and functions of genes become analyzable in detail. This paper outlines the analysis using this system by focusing on GATA-2, a transcription factor expressed in undifferentiated blood cells. |
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