Large-Scale Production of Porcine Mature Interleukin-18 (IL-18) in Silkworms Using a Hybrid Baculovirus Expression System.
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Accession number;03A0173302
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| Title;Large-Scale Production of Porcine Mature Interleukin-18 (IL-18) in Silkworms Using a Hybrid Baculovirus Expression System. |
| Author;
MUNETA Y
(National Inst. Animal Health, Ibaraki, Jpn)
ZHAO H K
(Shandong Agricultural Univ., Shandong, Chn)
INUMARU S
(National Inst. Animal Health, Ibaraki, Jpn)
MORI Y
(National Inst. Animal Health, Ibaraki, Jpn)
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Journal Title;J Vet Med Sci
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Journal Code:F0905A
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ISSN:0916-7250
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VOL.65;NO.2;PAGE.219-223(2003)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.16 |
| Pub. Country;Japan |
| Language;English |
| Abstract;In this report, a hybrid baculovirus expression system, which means a hybrid virus of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (IL-18) in silkworms. Two recombinant hybrid baculoviruses containing cDNA of the porcine precursor IL-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. After the co-infection of the two viruses, porcine mature IL-18 was efficiently produced in the haemolymph. The concentration of IL-18 in the haemolymph was 80-100 .MU.m/ml, as determined by porcine IL-18 specific ELISA. This yield was twenty-times more than that of the insect cell expression system described previously. The porcine mature IL-18 produced by the silkworms strongly induced interferon-.GAMMA. (IFN-.GAMMA.) production from porcine PBMC. An insect factory system for the large-scale production of useful cytokines for livestock animals will be available in the near future. (author abst.) |
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