The Effect of Terbutaline on Airway Epithelial Ion Transport in Sensitized Guinea Pig.
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Accession number;03A0140975
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| Title;The Effect of Terbutaline on Airway Epithelial Ion Transport in Sensitized Guinea Pig. |
| Author;
HATTORI TOMOHIRO
(Nihondai I Naika Naikaichi)
MAJIMA TOORU
(Nihondai I Naika Naikaichi)
HORIE TAKASHI
(Nihondai I Naika Naikaichi)
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Journal Title;Journal of the Japan Broncho-Esophagological Society
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Journal Code:Z0674A
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ISSN:0029-0645
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VOL.54;NO.1;PAGE.15-20(2003)
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| Figure&Table&Reference;FIG.3, REF.16 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Recently, the effect of cromolyn sodium (DSCG) on Cl channel blockers and the ion transport of airway epithelial cells have become a concern in cases of bronchial asthma. In this study, we examinee the change in ion transport in sensitized guinea pig tracheae with ovalbumin (OA) by the voltage clamp method. The guinea pigs were separated non-sensitized (NSE) and sensitized groups (SE), and the latter were sensitized by OA with alum. They were all anesthetized with pentobarbital sodium, and then tracheae were removed for physiological studies. Segments of the tracheae were mounted in Ussing chambers and bathed in Krebs-Henseleit (K-H) solution. The short-circuit current (Isc) and potential difference (PD) were monitored continuously. After Isc stabilization, terbutaline was changed from 1*10-5 to 1*10-2 M on the mucosal side after treatment with indomethacin (IND). Terbutaline (3*10-3 M) was added on the mucosal side after treatment with amiloride, diphenylamine-2-carboxylate (DPC), 4, 4-diisothiocyanato-stilbe-2, 2-disulfonic acid (DIDS), and furosemide. .DELTA.Isc showed a tendency to increased in both the NSE and SE groups after treatment with terbutaline at 1*10-2 M. .DELTA.Isc was suppressed significantly after treatment with DPC in both the NSE and SE groups (p<0.05). We concluded that the cyclic-AMP-dependent Cl channel was active in both the NSE and SE groups, and there was not a marked difference between the NSE and SE groups. (author abst.) |
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