Immobilization of Candida Cells Showing Mycelium-Like Shapes in Porous Polyvinyl Formal Resin and Their Applications.

Accession number;03A0164936
Title;Immobilization of Candida Cells Showing Mycelium-Like Shapes in Porous Polyvinyl Formal Resin and Their Applications.
Author; SHIOMI N (Kobe Coll., Nishinomiya, Jpn) INOUE Y (Kobe Coll., Nishinomiya, Jpn) TOMIOKA K (Kurume National Coll. Technol., Fukuoka, Jpn) YASUDA T (Kobe Univ., Kobe, Jpn)
Journal Title;J Chem Eng Jpn
Journal Code:S0629A
ISSN:0021-9592
VOL.36;NO.2;PAGE.161-165(2003)
Figure&Table&Reference;FIG.9, REF.11
Pub. Country;Japan
Language;English
Abstract;Candida rugosa ATCC14830 cells have high tendency to make flocculent masses by entanglement of the mycelium-like shaped cells and can be well immobilized in porous polyvinyl formal(PVF) resin. Moreover, Candida lipolytica cells, which became to show a mycelium-like shape by mutation, were also immobilized in PVF resin. These C. rugosa and mutant C. lipolytica cells were immobilized in PVF resins and cultured by the draw-fill method 2-3 times. These C. rugosa and C. lipolytica cells could decompose L-lactic acid and produce bio-surfactant liposan, respectively. Because high cell densities could be attained in immobilized systems, the total productivity in repeated-batch operations with immobilized cells was higher than those in batch operations with free cells. Moreover, the decomposed amount of L-lactic acid did not decrease during the repeated-batch operations, because the number of immobilized cells in PVF resin was kept almost constant. (author abst.)
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