Recent advances in cell-free protein synthesis: Application for postgenome sciences.

Accession number;03A0158592
Title;Recent advances in cell-free protein synthesis: Application for postgenome sciences.
Author; ENDO YAETA (Ehime Univ., Faculty of Engineering, JPN) SAWASAKI TATSUYA (Ehime Univ., Faculty of Engineering, JPN)
Journal Title;Protein, Nucleic Acid and Enzyme
Journal Code:F0325A
ISSN:0039-9450
VOL.48;NO.4;PAGE.549-554(2003)
Figure&Table&Reference;FIG.5, REF.12
Pub. Country;Japan
Language;Japanese
Abstract;It is an important problem toward post genome research to establish the technology to synthesize proteins freely based on genetic informations. Though conventional cell-free protein synthetic systems retain the properties of excellent rapidity and accuracy,their reaction systems are unstable, and have not been utilized for protein preparation. This article introduces both the stable and the high-efficient cell-free protein synthetic system developed by authors on the basis of elucidating unstabilization mechanism of wheat embryo buds extracts, and the practical wheat embryo buds cell-free protein synthetic system by integrating recently established elemental technologies. As these technologies do not utiilize processes of living organisms, they are expected to explore great possibility for not only biotechnology areas, but also scientific industries in the twenty-first century.
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