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Accession number;03A0366062
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| Title;Thrombolytic Activity of an Aqueous Extract of Tempe |
| Author;
SUMI HIROYUKI
(Kurashiki Univ. Sci. and the Arts, Coll. Sci. and Industrial Technol., JPN)
OKAMOTO TAKESHI
(Kurashiki Univ. Sci. and the Arts, Coll. Sci. and Industrial Technol., JPN)
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Journal Title;Journal of Home Economics of Japan
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Journal Code:F0763A
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ISSN:0913-5227
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VOL.54;NO.5;PAGE.337-342(2003)
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| Figure&Table&Reference;FIG.6, TBL.2, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;An aqueous extract of tempe exhibited strong thrombolytic activity, the average activity being about 450 IU (as urokinase)/g dry weight. The isoelectric point of the enzyme in tempe providing this thrombolytic activity was estimated to be about 8.7 by isoelectric focusing and its molecular weight to be about 30,000 by gel filtration. This enzyme not only digested thrombotic protein (fibrin) but also slightly digested Suc-Ala-Ala-Pro-Phe-pNA and H-D-Val-Leu-Lys-pNA. The activity of this enzyme was lost above 65.DEG.C. and greatly inhibited by 1 mM diisopropyl fluorophosphate and by 1 mg/ml of elastatinal. The inhibition of elastinal for the digestion of Suc-Ala-Ala-Pro-Phe-pNA was competitive and its Ki value was about 9.8*10-8M. (author abst.) |
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