Quantitative Analysis Method for Mumefural in Japanese Apricot Fruit Juice Concentrate
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Accession number;03A0324726
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| Title;Quantitative Analysis Method for Mumefural in Japanese Apricot Fruit Juice Concentrate |
| Author;
YADA HIROSHI
(National Food Res. Inst., JPN)
GATO NOBUKI
(Nakanobc)
NAGATOMO HIDENORI
(Umetanhompo)
CHUDA YOSHIHIRO
(Norinsuisanshohigijutsuse)
ONO HIROSHI
(National Food Res. Inst., JPN)
YOSHIDA MITSURU
(National Food Res. Inst., JPN)
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Journal Title;Journal of the Japanese Society for Food Science and Technology
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Journal Code:F0895A
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ISSN:1341-027X
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VOL.50;NO.4;PAGE.188-192(2003)
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| Figure&Table&Reference;FIG.4, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Mumefural is a component of Japanese apricot fruit juice concentrate, which has been used as a traditional nonofficial medicine in Japan, and was reported to have an activity to improve the blood fluidity. For quantitative analysis of mumefural, water extract of the fruit-juice concentrate was loaded on a cartridge column for solid-phase extraction (Oasis HLB,Waters) and eluted with 20% acetonitrile. Following HPLC analysis on a XTerra MS C18 column (Waters) by linear gradient elution with acetonitrile-20mM sodium phosphate buffer (pH2.3) gave a peak of mumefural at 280nm, from which area quantity of the compound could be elucidated based on the calibration curve obtained in this work. Mumefural content of 4 Japanese apricot fruit-juice concentrates was determined as 1.41mg/g, 0.361mg/g, 11.5mg/g, and 3.82mg/g, respectively, by this method. Accurate molar absorption coefficient (.EPSILON.) of mumefural was determined as 1.78*104 (.LAMBDA.max 282nm,water). Since mumefural is highly hygroscopic amorphous solid, it is difficult to weigh out an accurate quantity for preparation of a standard solution. Molar absorption coefficient of mumefural is, therefore, useful to calibrate the concentration of the standard solution for HPLC analysis. (author abst.) |
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