Studies on Estrogenic Activities of Food Additives with Human Breast Cancer MCF-7 Cells and Mechanism of Estrogenicity by BHA and OPP
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Accession number;03A0389551
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| Title;Studies on Estrogenic Activities of Food Additives with Human Breast Cancer MCF-7 Cells and Mechanism of Estrogenicity by BHA and OPP |
| Author;
OKUBO TOMOKO
(Tokyotokenkoanzenkenkyuse)
KANO ITSU
(Tokyotokenkoanzenkenkyuse)
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Journal Title;Journal of the Pharmaceutical Society of Japan
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Journal Code:F0508A
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ISSN:0031-6903
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VOL.123;NO.6;PAGE.443-452(2003)
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| Figure&Table&Reference;FIG.4, TBL.3, REF.33 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Estrogenic activities of more than 90 chemicals including food additives, foodstuffs of plant origin, and some chemicals, which could be orally ingested, were examined by assaying estrogen receptor (ER)-dependent proliferation of MCF-7 cells. Among 66 food additives, 17 compounds stimulated the proliferation, but their concentrations giving maximal cell yield were higher than that of 17.BETA.-estradiol and their estrogenic activities were weak. Flavonoids had relatively strong estrogenic activities. In the assay of ER competitive binding to human ER.ALPHA. and ER.BETA. in vitro, the antioxidant t-butylhydroxyanisole (BHA) had the capacity to compete with 17.BETA.-estradiol, while the capacity of o-phenyl phenol (OPP) was too small to calculate. Both BHA and OPP induced a decrease in gene expression of ER.ALPHA. and an increase in that of progesterone receptor in a time-dependent manner. These effects were similar to that of 17.BETA.-estradiol, a though much higher concentrations were required for these compounds than 17.BETA.-estradiol. These results may suggest that we should be careful not to ingest excessive food additives. (author abst.) |
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