Physiologic Role of Decidual .BETA.1 Integrin and Focal Adhesion Kinase in Embryonic Implantation
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Accession number;03A0337720
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| Title;Physiologic Role of Decidual .BETA.1 Integrin and Focal Adhesion Kinase in Embryonic Implantation |
| Author;
HANASHI H
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
SHIOKAWA S
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
AKIMOTO Y
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
SAKAI K
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
SAKAI K
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
SUZUKI N
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
NAGAMATSU S
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
IWASHITA M
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
NAKAMURA Y
(Kyorin Univ. School Of Medicine, Tokyo, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.50;NO.2;PAGE.189-198(2003)
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| Figure&Table&Reference;FIG.8, TBL.1, REF.48 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Implantation refers to a series of interactions between embryo and endometrium including hatching, attachment, and outgrowth. We investigated the expression and function of .BETA.1 integrin and focal adhesion kinase (FAK) in human decidual cells during implantation. Immunofluorescent staining localized .BETA.1 integrin to surfaces of cultured decidual cells. Double staining for .BETA.1 integrin and mediators of intracellular signaling involving .BETA.1 integrin, such as FAK and vinculin, colocalized .BETA.1 integrin with these substances, suggesting that human decidual cells express .BETA.1 integrin in the focal adhesion region. We next investigated the actions of .BETA.1 integrin and FAK in implantation by co-culturing mouse embryos and human decidual cells. Mouse blastocysts attached to cultured decidual cells after embryo hatching, usually within 24h of culture initiation. Blastocysts attached to decidual cells exhibited extensive outgrowth at 48h. Treatment of decidual cells with an antibody against .BETA.1 integrin or with an antisense FAK oligonucleotide did not affect hatching or attachment of blastocysts, but either one could inhibit outgrowth. Thus, it was concluded that human decidual .BETA.1 integrin and FAK participate in this final step of implantation. (author abst.) |
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