Evaluation of a Forced-air-ventilated Micro-isolation System for Protection of Mice against Pasteurella pneumotropica.
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Accession number;03A0376731
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| Title;Evaluation of a Forced-air-ventilated Micro-isolation System for Protection of Mice against Pasteurella pneumotropica. |
| Author;
HASEGAWA M
(Dai-dan Co., Ltd., Saitama, Jpn)
KAGIYAMA S
(Osaka Univ. Medical School, Osaka, Jpn)
TAJIMA M
(Osaka Univ. Medical School, Osaka, Jpn)
YOSHIDA K
(Dai-dan Co., Ltd., Saitama, Jpn)
MINAMI Y
(Osaka Univ. Medical School, Osaka, Jpn)
KUROSAWA T
(Osaka Univ. Medical School, Osaka, Jpn)
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Journal Title;Exp Anim
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Journal Code:Z0755A
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ISSN:1341-1357
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VOL.52;NO.2;PAGE.145-151(2003)
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| Figure&Table&Reference;FIG.2, TBL.1, REF.26 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Studies to date have established that the physical environment inside cages can be controlled adequately by setting the intra-cage ventilation at 60 air changes per hour in a forced-air-ventilated micro-isolation system (FVMIS). In this study, the capability of FVMIS to prevent inter-cage transmission of microorganisms was evaluated using Pasteurella pneumotropica as a reference microorganism. One FVMIS rack and a conventional rack were used, and cages with mice positive for P. pneumotropica and those with P. pneumotropica-free mice were housed on both racks. The mice were examined for P. pneumotropica contamination every 4 weeks after initiating the experiment for 12 weeks using a polymerase chain reaction method. Some P. pneumotropica-free mice housed in open air cages in the conventional rack became positive for P. pneumotropica (four of 28 animals after 4 weeks; eight of 28 animals after 12 weeks), but all P. pneumotropica-free mice housed in the FVMIS cages remained negative for the bacterium throughout the experiment. The results demonstrate that FVMIS can prevent inter-cage transmission of P. pneumotropica when proper cage handling practice is under taken. (author abst.) |
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