Evaluation of Developmental Competence of Vitrified-warmed Early Cleavage Stage Embryos and their Application for Chimeric Mouse Production.
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Accession number;03A0376737
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| Title;Evaluation of Developmental Competence of Vitrified-warmed Early Cleavage Stage Embryos and their Application for Chimeric Mouse Production. |
| Author;
KITO S
(National Inst. Radiological Sci., Chiba, Jpn)
NOGUCHI Y
(Sci. Services, Chiba, Jpn)
OHTA Y
(Sci. Services, Chiba, Jpn)
OHHATA T
(Chiba Univ., Chiba, Jpn)
ABE M
(National Inst. Radiological Sci., Chiba, Jpn)
SHIOMI N
(National Inst. Radiological Sci., Chiba, Jpn)
SHIOMI T
(National Inst. Radiological Sci., Chiba, Jpn)
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Journal Title;Exp Anim
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Journal Code:Z0755A
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ISSN:1341-1357
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VOL.52;NO.2;PAGE.179-183(2003)
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| Figure&Table&Reference;TBL.2, REF.22 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The developmental competence of in vitro cultured embryos vitrified-warmed at an early cleavage stage (2- or 4, 8-cell stage) was examined by both direct transfer into recipient animals and after in vitro manipulation for chimeric mice production using embryonic stem (ES) cells. Vitrified-warmed embryos transferred at the morulae and blastocyst stages showed fetus development comparable to control embryos, although blastocyst development of vitrified-warmed embryos was significantly slower than that of controls. When vitrified-warmed early cleavage stage embryos were used for chimeric mouse production using ES cells, 1 to 10% of the injected or aggregated embryos developed into chimeric neonates and germ-line chimeric mice were obtained from all ES cell lines. This study indicates that embryos developed in vitro from vitrified-warmed embryos have equivalent competence with unvitrified embryos irrespective of stage of vitrification and that these vitrified-warmed embryos maintain adequate viability even after in vitro manipulation such as aggregation and microinjection with ES cells. (author abst.) |
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