Molecular characterization of NADPH oxidase1 expressed on large intestinal epithelial cells
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Accession number;03A0408544
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| Title;Molecular characterization of NADPH oxidase1 expressed on large intestinal epithelial cells |
| Author;
KUWANO YUKI
(Univ. of Tokushima, Sch. of Med.)
KAWAHARA TSUKASA
(Univ. of Tokushima, Sch. of Med.)
KODAMA NANAE
(Univ. of Tokushima, Sch. of Med.)
NAKAI SAORI
(Univ. of Tokushima, Sch. of Med.)
KISHI KYOICHI
(Univ. of Tokushima, Sch. of Med.)
ROKUTAN KAZUHITO
(Univ. of Tokushima, Sch. of Med.)
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Journal Title;Shikoku Acta Medica
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Journal Code:G0586A
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ISSN:0037-3699
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VOL.59;NO.1/2;PAGE.45-46(2003)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;NADPH oxidase 1 (Nox1) is an isozyme of gp91-phox predominantly expressed in the human colon. In this study, we have established primary cultures of guinea pig large intestinal epithelial cells (LIEC). A great majority of the cultured cells (>90%) was surface mucous cells containing periodic acid-Schiff reaction-positive granules. Vimentin-positive fibroblasts were <1%, and macrophages were not contaminated. LIEC spontaneously produced superoxide anion (O2-) at about 160 nmol/mg protein/h. O2--dependent formation of blue formazan particles from nitroblue tetrazolium was observed only on surface of mucous-producing cells, and these cells expressed Nox1 protein at plasma membrane and in the cytoplasm. They expressed p67-phox, p22-phox, and rac1, but not gp91-phox, p47-phox, p40-phox, and rac 2. Immunohistochemistry showed that Nox1, p67-phox, and p22-phox were predominantly expressed in surface mucous cells of human and guinea pig colonic mucosa. Human colon cancer cell lines (Caco2, T84, and HT29 cells) expressed Nox1, p22-phox, and rac1, but not the other NADPH components. These cells secreted O2- at <5 nmol/mg protein/h. Caco2 cells possessed Toll-like receptor 5, and flagellin (FliC) from Salmonella enteritidis phosphorylated transforming growth factor-.BETA.-activated kinase 1 (TAK1) and TAK1-binding protein 1, and significantly up-regulated O2- production. These results suggest that Nox1 expressed in colonic epithelial cells may regulate interactions between pathogenic bacteria and epithelial cells for host defense. (author abst.) |
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