Spontaneous Hydrogen Production from Wheat Bran
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Accession number;04A0015260
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| Title;Spontaneous Hydrogen Production from Wheat Bran |
| Author;
TAGUCHI FUMIAKI
(Kitasato Univ., Graduate School of Medical Sci., JPN)
YANO REIKO
(Kitasato Univ., Graduate School of Medical Sci., JPN)
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Journal Title;Journal of the Japan Society of Waste Management Experts
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Journal Code:L1596A
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ISSN:
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VOL.14;NO.6;PAGE.329-333(2003)
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| Figure&Table&Reference;FIG.2, TBL.2, REF.7 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;This paper explains the following items on the titled technology: 1) Background; hydrogen was generated from wheat bran (I) when it was stored at 37 .DEG.C. overnight in wetting state with de-ionized water, 2) results of detailed study on spontaneous hydrogen generation conditions from I; foaming was observed after about 6 hours of warming, followed by abrupt increase in gas generation amount, when 40 g of I and 15 g of trypticase soy broth powder were suspended in 500 mL of de-ionized water and kept in a sealed container at 43 to 45 .DEG.C.. Maintaining the culture liquid under pH 5.2 provided maximal hydrogen generation of about 2,400 mL in total and weight of I decreased from initial 40 g by about 10 g after hydrogen fermentation. These results revealed hydrogen gas production of 60 mL/g and 240 mL/g based on I and material consumed, respectively, 3) results of another test, wherein 10 g of I (or 5 g of glucose as a control test) was added to 500 mL of PY culture media, followed by sterilization and mixed with hydrogen-producing bacteria strain AM21B in an anaerobic glove box, sealing with a rubber stopper and incubation at 37 .DEG.C. under pH 5.7; hydrogen generation of 1,328 mL and 1,776 mL from I and control, respectively. These results showed 10 g of I is equivalent to 3.75 g of glucose in hydrogen production and 4) gas composition from I; hydrogen about 70 %, carbon dioxide about 28 % and each of nitrogen and oxygen about 2 %. |
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