Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents
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Accession number;04A0042347
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| Title;Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents |
| Author;
MAHAKUNAKORN P
(Toyama Medical And Pharmaceutical Univ., Toyama, Jpn)
TOHDA M
(Toyama Medical And Pharmaceutical Univ., Toyama, Jpn)
MURAKAMI Y
(Toyama Medical And Pharmaceutical Univ., Toyama, Jpn)
MATSUMOTO K
(Toyama Medical And Pharmaceutical Univ., Toyama, Jpn)
WATANABE H
(Toyama Medical And Pharmaceutical Univ., Toyama, Jpn)
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Journal Title;Biol Pharm Bull
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Journal Code:S0989A
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ISSN:0918-6158
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VOL.27;NO.1;PAGE.38-46(2004)
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| Figure&Table&Reference;FIG.6, REF.36 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The antioxidant properties of Choto-san and its related constituents such as Chotoko and Choto-san without Chotoko, and phenolic compounds contained in Chotoko such as epicatechin, caffeic, acid and quercetin were evaluated. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, the scavenging activity of Chotoko (IC50 14.3 .MU.g/ml) was found to be higher than that of Choto-san (IC50 206.2 .MU.g/ml) and Choto-san without Chotoko (IC50 244.3 .MU.g/ml). Epicatechin (IC50 10.4 .MU.M), caffeic acid (IC50 13.8 .MU.M), and quercetin (IC50 7.1 .MU.M) also revealed scavenging activity against DPPH radicals. Choto-san (IC50 67.7 .MU.g/ml) exhibited stronger inhibitory activity against superoxide anion formation than Choto-san without Chotoko (IC50 92.4 .MU.g/ml) but weaker activity than Chotoko (IC50 18.3 .MU.g/ml). The generation of superoxide anion was also inhibited by epicatechin (IC50 175.2 .MU.M), caffeic acid (IC50 141.7 .MU.M), and quercetin (IC50 18.7 .MU.M). In a hydroxyl radical-scavenging experiment, Choto-san (IC50 2.4 mg/ml), Chotoko (IC50 2.2 mg/ml), Choto-san without Chotoko (IC50 2.8 mg/ml), epicatechin (IC50 3.9 mM), caffeic acid (IC50 3.6 mM), and quercetin (IC50 1.9 mM) exhibited activity. In NG108-15 cells, when added simultaneously with H2O2 (500 .MU.M), Choto-san (250 .MU.g/ml), Chotoko (250 .MU.g/ml), Choto-san without Chotoko (500 .MU.g/ml), epicatechin (200 .MU.M), caffeic acid (200 .MU.M), and quercetin (200 .MU.M) effectively protected cells from oxidative damage. In conclusion, the present results provide evidence that Choto-san acts as an antioxidant and cytoprotective agent against oxidative damage, which is due at least partly to the phenolic compounds contained in Chotoko. (author abst.) |
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