Establishment of an Enzyme-Linked Immunosorbent Assay for Measurement of Sotalol
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Accession number;04A0042357
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| Title;Establishment of an Enzyme-Linked Immunosorbent Assay for Measurement of Sotalol |
| Author;
SAITA T
(Saga Medical School, Saga, Jpn)
FUJITO H
(Saga Medical School, Saga, Jpn)
NAKANO Y
(Saga Medical School, Saga, Jpn)
MORI M
(Saga Medical School, Saga, Jpn)
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Journal Title;Biol Pharm Bull
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Journal Code:S0989A
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ISSN:0918-6158
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VOL.27;NO.1;PAGE.94-96(2004)
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| Figure&Table&Reference;FIG.2, TBL.3, REF.17 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We have established an enzyme-linked immunosorbent assay suitable for routine monitoring of serum levels of sotalol. Anti-sotalol antibody was obtained by immunizing rabbits with sotalol conjugated with bovine serum albumin using the N-succinimidyl ester method. An enzyme marker was similarly prepared by coupling sotalol with .BETA.-D-galactosidase. The detection limit of sotalol by the enzyme-linked immunosorbent assay was approximately 32 ng/ml with 50-.MU.l samples. This assay was specific for sotalol because of very slight cross-reactivity with 4-(methanesulfonylamino)benzonitrile (1.6%), but none with D,L-isoproterenol. Using this assay, drug levels were easily measured in the serum of rabbits after oral administration of sotalol at a single dose of 3 mg/kg. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of sotalol. (author abst.) |
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