Susceptibility of Mouse Splenic Cells to Oxidative DNA Damage by X-Ray Irradiation
|
Accession number;04A0042360
|
| Title;Susceptibility of Mouse Splenic Cells to Oxidative DNA Damage by X-Ray Irradiation |
| Author;
TAHARA S
(Tokyo Metropolitan Inst. Of Gerontology, Tokyo, Jpn)
KANEKO T
(Tokyo Metropolitan Inst. Of Gerontology, Tokyo, Jpn)
|
Journal Title;Biol Pharm Bull
|
Journal Code:S0989A
|
ISSN:0918-6158
|
|
VOL.27;NO.1;PAGE.105-108(2004)
|
| Figure&Table&Reference;FIG.3, REF.27 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Susceptibility to oxidative stress by X-ray irradiation was examined in splenic cells of BDF1 mouse and fetal human lung fibroblasts, TIG-7. Survival rates of splenic cells irradiated with X-rays were lower than those of TIG-7 cells irradiated similarly. The content of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) immediately after X-ray irradiation in the DNA of splenic cells increased until 2 Gy irradiation, but remained constant above 2 Gy. The 8-oxodG contents rose in proportion to the dose of X-rays in TIG-7 cells. Although the survival rate of splenic cells exposed to 1 Gy irradiation decreased with time, the survival rate of TIG-7 cells remained unchanged. The 8-oxodG content in splenic cells irradiated with X-rays did not decrease even 48 h after irradiation, while that in TIG-7 cells decreased with time, and recovered to the pre-irradiation level after 48 h. A DNA ladder was observed in splenic cells 2 h after X-ray irradiation, but the ladder was not found in fibroblasts. Furthermore, caspase-3 activity increased after X-ray irradiation of splenic cells. These results indicate that splenic cells are sensitive to oxidative stress induced by X-ray irradiation and that splenic cells damaged by even low doses of X-rays are removed through apoptosis rather than by a repair pathway. (author abst.) |
|
|
|
Related Articles;
|
|
|
