Study on Genes Promoting Trophoblast Differentiation in Mammalian Embryos

Accession number;04A0022314
Title;Study on Genes Promoting Trophoblast Differentiation in Mammalian Embryos
Author; KOBAYASHI MASAYUKI (Akita Prefectural Univ., Fac. Bioresource Sci., JPN)
Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
Journal Code:X0296A
ISSN:
VOL.21;NO.;PAGE.16-20(2003)
Figure&Table&Reference;FIG.1, TBL.1, REF.8
Pub. Country;Japan
Language;Japanese
Abstract;Cloning from somatic cells is a powerful strategy for reproduction of animals expressing desirable genetic characters. However, several abnormal phenotypes have often been observed especially in the placental morphogenesis of the cloned cattle and mice. Thus the productivity of live cloned animals have been lowered. In order to improve the productivity by leading appropriate morphogenesis of placenta during the development of cloned animals, it is necessary that the identification of master genes regulating the trophoblast differentiation, which is the first step of the placental morphogenesis, at the compact morula stage. In this study, the developmental stage-specific subtracted cDNA libraries generated from mouse 4 to 8-cell embryos, compact morulae and early blastocysts were constructed by the PCR-based suppression subtraction hybridization(SSH) method. The development stage-specific cDNA clones in the libraries were selected by means of the differential screening. About 25% of cDNA clones were judged as the positive. Nucleotide sequences of the positive clones were determined and analyzed by the blast search to estimate functions of corrsponding genes. Several cDNA clones were classified into metabolic enzymes, growth factors or transcription factors. RT-PCR analysis revealed that the expression of these genes oscillated during the development of preimplantation mouse embryos. It is necessary that the clarification of the function of these genes during embryonic developoment. (author abst.)
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