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Accession number;04A0022315
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| Title;Studies on the Production of Somatic Cell Cloned Pigs |
| Author;
TSUNODA YUKIO
(Kinki Univ., Faculty of Agriculture, JPN)
KATO YOKO
(Kinki Univ., Faculty of Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.21-23(2003)
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| Figure&Table&Reference;REF.4 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In our previous report, we demonstrated that brief treatment of MII porcine oocytes with 0.4.MU.g/ml demecolcine in the presence of 0.05M sucrose results in a membrane protrusion that contains a condensed chromosome mass. The present study examined the optimal conditions for demecolcine and nocodazole treatment in the chemically-assisted removal of chromosomes. When matured oocytes were treated with 0.1 to 0.4.MU.g/ml demecolcine for 60min and with 0.4.MU.g/ml for 30min or 3.MU.g/ml nocodazole for 30 and 60min, more than 70% of oocytes had a membrane protrusion in which condensed chromosomes were located. The in vitro developmental ability of enucleated oocytes assisted by 0.1 and 0.4.MU.g/ml demecolcine or 3.MU.g/ml nocodazole receiving porcine somatic cells was not significantly different. After transfer to 10 recipiens, however, two of six recipients that receiving demecolcine-treated enucleated eggs produced four healthy cloned piglets but none of the four recipients of nocodazole-treated enucleated eggs produced piglets. Further studies are required to increase the successful development to term because the proportion of live piglets was still low(4/339, 1.2%). (author abst.) |
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