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Accession number;04A0022319
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| Title;Study on the Development of the Long-term Preservation and Transportation Method for the Bovine Ovaries and Oocytes |
| Author;
DOUCHI OSAMU
(Rakuno Gakuen Univ.)
IMAI KEI
(National Inst. Agrobiological Sci.)
KANEYAMA KANAKO
(National Livestock Breeding Center, JPN)
NAGAI TAKASHI
(National Inst. Agrobiological Sci.)
SAITO NORIO
(National Livestock Breeding Center, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.39-45(2003)
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| Figure&Table&Reference;TBL.6, REF.23 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The objective of this study was to study the effects of different method of the long-term preservation for ovaries and immature oocytes on the development of in vitro produced embryos in bovine. In experiment 1, evaluated the effects of different ways of storing bovine ovaries on the development and quality of blastocyst in vitro produced embryos. Bovine ovaries obtained at a local abattoir were kept in D-PBS(PBS), normal saline(Saline), and a plastic bag(Bag) for 15-18h at 25.DEG.C.. The cleavage rate of the PBS(61.5%) was significantly lower than the Bag(78.6%)(p<0.05). The blastocyst rate of the control(28.2%) tended to be higher than the PBS(18.3%)(p<0.1). The cell number in the blastocyst(mean.+-.SE) of the control(173.1.+-.9.3) was significantly higher than that of the Saline(137.3.+-.11.5)(p<0.05), and that of the PBS(172.6.+-.16.4) tended to be higher than the Saline(p<0.1). In experiment 2, evaluated the effects of butyrolactone-(BL-I) and cycloheximide(CHX) on germinal vesicle breakdown(GVDB) in bovine oocytes and subsequent in vitro development after in vitro maturation and fertilization. Bovine cumulus oocyte complex(COCs) were collected by the aspiration of 3-5mm follicles of ovaries obtained at a local abattoir. The COCs were preincubated for 24h in TCM-199 supplemented with 100mM BL-I and 3mg-ml BSA or 100.MU.l/ml CHX and 5% CS. There were no differences in the rates of the oocytes at the GV stage between BL-I and CHX, but the rates of the oocytes at the M II stage of BL-I tended to be lower than CHX(p<0.1). There were no differences in sperm penetration, normal fertilization, and polyspermy after in vitro maturation and fertilization. The cleavage rate of CHX(81.0%) was significantly higher than BL-I(65.0%)(p<0.01), and the control(75.5%) tended to be higher than BL-I(p<0.1). The blastocyst rate of control(32.0%) was significantly higher than the BL-I(19.2%)(p<0.05).... (author abst.) |
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