Science Links Japan | Study on the Application of Cytokines in the Regulation of Estrous Cycle and Reproduction of Cattle

Study on the Application of Cytokines in the Regulation of Estrous Cycle and Reproduction of Cattle

Accession number;04A0022320

Title;Study on the Application of Cytokines in the Regulation of Estrous Cycle and Reproduction of Cattle

Author; OKUDA KIYOSHI (Okayama Univ., Graduate School of Natural Sci. and Technol., JPN) MURAKAMI SHUKO (Okayama Univ., Graduate School of Natural Sci. and Technol., JPN)

Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho

Journal Code:X0296A

ISSN:

VOL.21;NO.;PAGE.46-52(2003)

Figure&Table&Reference;FIG.4, TBL.1, REF.33

Pub. Country;Japan

Language;Japanese

Abstract;Tumor necrosis factor-.ALPHA.(TNF.ALPHA.) has been suggested by recent in vitro studies to play a role in the initiation of luteolysis in cattle. On the other hand, TNF.ALPHA. also induced the production and output of luteotropic PGE2 in bovine corpus luteum(CL) and endometrium, suggesting luteotropic and luteoprotective roles of TNF.ALPHA.. However, the properties and mechanisms of the opposite TNF.ALPHA. actions(luteolytic versus leuteotropic) during the estrous cycle and pregnancy are not yet clarified. The aim of the present study was to examine the influence of TNF.ALPHA. on the estrous cycle in cattle, by observing secretory function of both the CL and the uterus as well as standing behavior. We evaluated the secretion of progesterone(P4), oxytocin(OT), nitric oxide(NO), and luteolytic(prostaglandin F2.ALPHA.-PGFM) and luteotropic(PGE2) metabolites of arachidonic acid in peripheral blood plasma, as parameters of TNF.ALPHA. action. Mature Holstein/Polish Black and White heifers(n=36) were infused on Day 14 of the estrous cycle(Day 0=estrus) for 30min into aorta abdominalis with saline, analogue of PGF2.ALPHA.(cloprostenol; 100.MU.g), or saline with TNF.ALPHA. in doses of 0.1, 1, 10, 25 or 50.MU.g per animal. Lower doses of TNF.ALPHA.(0.1 and 1.MU.g/animal) decreased P4 levels in blood plasma, and consequently resulted in shortening the estrous cycle(18.8.+-.0.9 and 18.0.+-.0.7 days, respectively, as compared to the control -22.3.+-.0.3 days; P<0.05). One microgram of TNF.ALPHA. increased PGFM(P<0.001) and NO(P<0.001) concentrations and decreased P4 levels in the blood plasma. Higher doses of TNF.ALPHA.(10,25,50.MU.g) stimulated synthesis of P4(P<0.001) and PGE2(P<0.001), and consequently resulted in prolongation of the estrous cycle(over 30 days; P<0.05). TNF.ALPHA. in luteolytic dose(1.MU.g) strongly decreased concentrations of OT in the peripheral blood(P<0.01), suggesting that OT is down-regulated during cytokine-dependent lueolysis.... (author abst.)

Related Articles;