Development of Molecular Probes Utilized to Identify Bovine Genetic Stocks

Accession number;04A0022322
Title;Development of Molecular Probes Utilized to Identify Bovine Genetic Stocks
Author; YAMAMOTO HIROAKI (Graduate School of Life Sci., Tohoku Univ., JPN) SATO SEIJI (Ito Ham Foods Inc., Central Res. Inst., JPN)
Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
Journal Code:X0296A
ISSN:
VOL.21;NO.;PAGE.57-58(2003)
Figure&Table&Reference;
Pub. Country;Japan
Language;Japanese
Abstract;To develop an easy method utilized to idnetify each individual cattle genetically is quite important to benefit both of the original stock(strain) developers and consumers who can trace the distribution channel of meat sold at a store. We have tried to survey appropriate genes easily applicable as genetic markers. We first focused on a gene responsible for coat color expression. Coat color is a visible marker controlled by many genes and is one of ideal phenotypes used for the first screening of a stock as a noninvasive method. To amplify genes covering most of its coding sequence by a PCR method, we surveyed pigmentation genes having small genomic sizes. DNA samples from five different bovine breeds maintained by Itoham Foods Inc were collected and used as templates. Several polymorphic sites were found in a pigmentation gene. Detailed analysis is now underway. (author abst.)