Comparison of Proteome Maps of Muscle Protein between Cattle Breeds
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Accession number;04A0022324
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| Title;Comparison of Proteome Maps of Muscle Protein between Cattle Breeds |
| Author;
MANNEN HIDEYUKI
(Kobe Univ., Fac. Agriculture, JPN)
OKADA MICHIO
(Kobe Univ., Fac. Agriculture, JPN)
TSUJI SOICHI
(Kobe Univ., Fac. Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.64-67(2003)
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| Figure&Table&Reference;FIG.2, REF.7 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The Japanese Balck cattle are valued for its highly marbled meat, while Holstein cattle and most famous for dairy cattle in the world. Both breeds are used for beef meat in Japan, however the meat qualities are quite different between these breeds. These are inherited traits, but the candidate proteins or genes controlled the traits are still unknown. Recently, in addition to genomic and transcriptomics, proteomics is attracted the attention for post-genome candidates. In this study, we carried out proteome analysis of bovine muscle protein. Two dimensional proteome map revealed approximately 1,300 protein spots. Five to ten spots showed quite strong signal, suggesting that these are proteins comprising of actins, myosin or collagens. Most of the spots located middle range of pH gradient and middle molecular weight. Comparison of the muscle protein profile between cattle breeds, which were age-matched animal fed identical diets, revealed 17 breed specific spots and 5 different signal intense spots, which suggested differences of protein expression level. Analyses using more samples and materials having high-resolution power would be required in order to identify the spots associated with economical traits or breed specific protein. (author abst.) |
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