Studies on Governmental Regulation in EU, and Development of Transgenic Mice against BSE
|
Accession number;04A0022325
|
| Title;Studies on Governmental Regulation in EU, and Development of Transgenic Mice against BSE |
| Author;
ONODERA TAKASHI
(Univ. of Tokyo, Fac. of Agric.)
|
Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
|
Journal Code:X0296A
|
ISSN:
|
|
VOL.21;NO.;PAGE.68-72(2003)
|
| Figure&Table&Reference;FIG.1, REF.6 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Transgenic(Tg) mice are among the most useful research tools in the field of prion biology. Especially, overexpression of the normal cellular form of prion protein(PrPc) may lead to the formation of PrPSc-like pathologic lesions, such as vacuolation. Also, even a limited overexpression may be lethal to founder Tg mice. Here we describe the pathological abnormalities of generated founder Tg mice by restricted transgene expression, which was observed during the development of prion agent susceptible Tg mice. Tg mice expressing the scimitar-horned oryx(Oryx dammah) PrP(OrPrP) gene were generated using chicken .BETA.-actin promoter and CMV enhancer, which were known to direct a widespread gene expression. As expected, transgene expression was observed ubiquitously in a nerve cell, an immunity cell and skeletal muscles. However, two of four founder mice showed abnormal sudden death during the generation of Tg(OrPrP) mice. In histopathology from this one sudden death founder, atropy was observed in the pyramidal cell layer of hippocampus. The other dead founder mouse showed hyaline degeneration in femur skeletal muscle. Two survived founder mice produced littermates. Histological examination of these two mice showed no significant abnormalities in brain and skeletal muscle, but multiple vacuolation was seen in the heart muscle. These data suggest that restricted OrPrP expression in Tg(OrPrP) mice may lead to sudden death or pathological abnormalities of tissues. (author abst.) |
|
|
|
Related Articles;
|
|
