Development of a New Genetic Diagnosis for Differentiation of Field Bovine Viral Diarrhea Viruses
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Accession number;04A0022327
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| Title;Development of a New Genetic Diagnosis for Differentiation of Field Bovine Viral Diarrhea Viruses |
| Author;
AKASHI HIROOMI
(Univ. Tokyo, Graduate School of Agricaltural and Life Sci., JPN)
TOOYA YUKINOBU
(Univ. Tokyo, Graduate School of Agricaltural and Life Sci., JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.79-83(2003)
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| Figure&Table&Reference;REF.18 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Infection with bovine viral diarrhea virus(BVDV) causes economic losses in cattle-producing areas around the world, and is considered one of the most significant pathogens of cattle. To complete the genetic characterization and establish the diagnosis method of the Japanese BVDV, we determined the full genome sequence of Nose strain. The full genome size of Nose strain was 13,196 nucleotides. The single large open reading frame encodes a polyprotein of 4,197 amino acids. An insertion of cellular sequence was found in the capsid(C) protein coding sequence in the Nose strain genomic RNA. A duplication of Npro and C sequences was also found downstream of the cellular sequence insertion. To our knowledge, this is the first report describing an insertion of a cellular sequence in the structural protein coding region of BVDV. According to the Nose and reported BVDV genomic sequences, we synthesized a primer pair for the E2 gene. Phylogenetic analysis of he 5' non-coding region(5'NCR) which has been used for the Pestivirus diagnosis and the E2 region of 48 Japanese and reported BVDV genome was performed. It has been reported that the E2 gene encodes glycoprotein that appears to be a major antigen reacting with virus neutralizing antibodies, and the E2 gene region is a more variable site. Similar subgroupd clustering of the Japanese isolates was found in the phylogenetic tree of each region, suggesting that mutation in both regions might be uniform. However, some isolates were classified in a different cluster in each genetic region. Thus the combination of the phylogenetic analysis of several regions was useful for characterization of Japanese BVDV. (author abst.) |
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