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Accession number;04A0022328
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| Title;Study on the Drug Therapy for Genetic Hemorrhagic Tendency in Japanese Black Cattle |
| Author;
ITO KATSUAKI
(Miyazaki Univ., Faculty of Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.84-88(2003)
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| Figure&Table&Reference;FIG.3, REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In order to treat a genetic hemorrhagic disease in cattle, it is important to know the aggregation mechanism of bovine platelets since the aggregation mechanism is quite different among animal species. In this stuffy we characterized the aggregation of bovine, human and rat platelets induced by the most physiological aggregation stimulant collagen. Bovine platelets showed less sensitivity to collagen than human or rat platelets. Collagen mobilizes endogenous ADP and thromboxane A2(TXA2) and these substances in turn potentiate the action of collagen. This study revealed that TXA2 was scarcely involved in aggregation of bovine platelets whereas it played a great role in the collagen-induced aggregation of human platelets. In fact, bovine platelets were insensitive to TXA2. On the other hand, contribution of endogenous ADP to the collagen-induced aggregation was greater in human and rat platelets than in bovine ones. To determine whether smaller contribution of ADP to the response to collagen in bovine platelets is ascribed to less release of ADP from dense granules than in human or rat platelets, we measured the efflux of [3H]-5-hydroxytryptamine([3H]-5-HT), which had been loaded in platelets, as an index of liberation response from dense granules. The amount of [3H]-5-HT liberated by collagen was slightly less in bovine platelets at intermediate concentrations than in human or rat ones. It is suggested that human platelets are more sensitive to ADP than bovine platelets. Experiments using the inhibitors of protein kinase C and phosphatidylinositol 3-kinase suggested that contribution of these kinases in the release response is different between human and bovine platelets.... (author abst.) |
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