Detection of Bovine and Swine Leptospira Infection Using a Recombinant Outer Membrane Protein, OmpL1
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Accession number;04A0022329
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| Title;Detection of Bovine and Swine Leptospira Infection Using a Recombinant Outer Membrane Protein, OmpL1 |
| Author;
OKUDA MASARU
(Yamaguchi Univ., Fac. Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.89-94(2003)
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| Figure&Table&Reference;FIG.4, REF.4 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;OmpL1 is a 31kDa outer membrane protein(OMP) characterized in 1993 and known to be expressed only in pathogenic Leptospira spp. Only pathogenic Leptospira in Japan is Leptospira interrigans. Because pathogenicities of Leptospira are different in serotypes, the genetic classification of Leptospira strains of pathogenic serovars is very useful for judgment in prognosis of leptospirosis. Detection of the specific antibodies by latex agglutination test is widely used for diagnosis of leptospirosis. However, this method is lacking in objectivity for the judgment and is difficult to deal with many samples at once. Therefore, establishment of a more sensitive and useful assay is required. In the present study, OmpL1 nucleotide and amino-acid sequences were compared in 5 main pathogenic Leptosipra serotypes in Japan(L.i. serovar icterohaemorrhagiae strain RGA, L.i. serovar canicola strain Ut-4, L.i. serovar autumnalis strain Akiyami A, L.i. serovar hebdmadis strain Akiyami B and L.i. serovar australis strain Akiyami C) and their phylogenic relationships were analyzed. It was revealed that the phylogenic relationship based on OmpL1 genes was more divergent than that of 23S ribosomal DNAs. Recombinant OmpL1(GST-rOmpL1) was expressed to establish the detection system of anti-Leptospira antibodies by ELISA using GST-rOmpL1 as an antigen. In immunoblot analysis, the protein reacted with sera infected by three different serotypes of Leptospira interrogens, and did not react with negative controls or sera infected with Borrlia burgdorferi of which pathogen is closely related with Leptospira.... (author abst.) |
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