Monitoring of Verotoxin-producing Escherichia coli in Cattle Kown as Healthy Carriers
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Accession number;04A0022330
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| Title;Monitoring of Verotoxin-producing Escherichia coli in Cattle Kown as Healthy Carriers |
| Author;
KOBAYASHI YASUO
(Hokkaido Univ., Graduate School of Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.95-98(2003)
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| Figure&Table&Reference;TBL.1, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;PCR-aided monitoring of verotoxin-producing Escherichia coli(VTEC) was performed over the period of 12 months by using monthly collected fresh feces from 5 dairy cows that had been identified as healthy VTEC carriers. PCR products were confirmed to be originated from verotoxin genes by Southern hybridization using a gene fragment of verotoxin 2 as a probe. Although seasonal variation of VTEC shedding seemed to be dependent of individual cow, the detection results suggested the possible influential factors as follows. Grazing, summer or outdoor rearing, during the period from the middle of May up to the beginning of October, may reduce VTEC shedding. Especially during the period from July to September, all 5 cows were VTEC-negative except a cow in August. Drying for milking cow was also suggested to have a depressive effect on VTEC shedding, i.e. 3 of 4 drying cows showed no shedding of VTEC. Meanwhile, winter or indoor rearing is pointed out to be a stimulating factor, i.e. 4 cows shed VTEC druing the period from November to April, excepting 5 cases of total 24 samples. More detailed tracking of VTEC is apparently necessary to identify what factors are significant and how their interrelationships are. (author abst.) |
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