Control of Salmonella Food Poisoning: Development of protective vaccine using chicken monoclonal antibodies
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Accession number;04A0022332
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| Title;Control of Salmonella Food Poisoning: Development of protective vaccine using chicken monoclonal antibodies |
| Author;
SASAI KAZUMI
(Osaka Prefecture Univ., Graduate School of Agriculture and Biological Sci., JPN)
TANI HIROYUKI
(Osaka Prefecture Univ., Graduate School of Agriculture and Biological Sci., JPN)
MATSUBAYASHI MAKOTO
(Osaka Joshi-Gakuen Jr. Coll.)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.104-107(2003)
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| Figure&Table&Reference;REF.15 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In spite of performing various measures, poultry and poultry products have been incriminated in the majority of traceable food-borne illnesses caused by Salmonella Enteritidis(following, SE). It is one of the most effective strategies to vaccinate chickens against it. To develop a vaccine against a pathogen, one of the available methods is to use the monoclonal antibody. In general, there were many research which used the mouse monoclonal antibody system. However, there has been no report of production of the chicken monoclonal antibody to SE. Since ten kinds of chicken monoclonal antibodies to SE(phage type 4, Y-24 strain) named CMo-1,CMo-2,CMo-3,CMo-4,CMo-5,CMo-6,CMo-7,CMo-8,CMo-9 and CMo-10 were obtained, characterizarion of SE antigen recognized with them and the cross-reactivity with 5 other sero-types of Salmonella, such as S. Typhimurium(following, ST), S. Infantis, S. Heidelberg, S. Hadar, and S. Montevideo were examined. Although these monoclonal antibodies have not recognized ST antigen, the cross-reactivity of 4 remaining sero-types were observed by the immunofluorescence assay. These chicken monoclonal antibodies were found cross-reactivity with several sero-types of Salmonella. Thus these antigens recognizied by them were expressed in common antigen sites and they could be a strong candidate for immunodiagnosis and vaccine development. (author abst.) |
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