Effects of Cytokines on the Production of IgA Receptor in Porcine Mammary Cells
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Accession number;04A0022335
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| Title;Effects of Cytokines on the Production of IgA Receptor in Porcine Mammary Cells |
| Author;
SAYAMA KAZUTOSHI
(Shizuoka Univ., Fac. Agriculture, JPN)
HANZAWA SHINJI
(Shizuoka Univ., Fac. Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.117-123(2003)
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| Figure&Table&Reference;FIG.3, REF.10 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;To clarify the mechanism of polymeric immunoglobulin receptor(pIgR) expression in porcine mammary gland, porcine mammary epithelial cells were cultured in vitro and effects of prolactin and several cytokines on the production of pIgR in the cells were examined. Porcine mammary cells were obtained from the mammary gland in 6-month-old virgin females by enzyme dissociation. Collected porcine mammary cells were incubated on floating collagen gel with the various culture mediums containing Insulin, Prolactin and cytokines such as Interleukin-4(IL-4), Interleukin-1.BETA.(IL-1), Interferon-.GAMMA.(IFN) and Tumor Necrosis Factor-.ALPHA.(TNF) singly or in combination. After the incubation, quantitative analysis of pIgR mRNA in the cells was performed by quantified PCR method. Moreover, secretion of secretary component(SC) from the cells to the medium after the culture was also analyzed by using western blotting method. As a result, expression level of pIgR mRNA increased in I+PRL, I+PRL+IL-4, I+PRL+IL-1, I+PRL+IFN, I+PRL+TNF, I+PRL+IL-4+IL-1, I+PRL+IL-4+IFN, I+PRL+IL-4+TNF, I+PRL+IL-1+IFN, I+PRL+IL-1+TNF and I+PRL+IFN+TNF groups; by 0.55, 13.14, 0.71, 5.50, 9.93, 11.03, 9.82, 5.12, 6.20, 2.53 and 3.80 times, respectively compared with it in I group. These results indicated that pIgR mRNA in cultured porcine mammary cells was expressed by I only, and the expression level were not increased by PRL. Moreover, it was clarified that the expression of pIgR mRNA were increased by IL-4, IFN and TNF. Particularly, the expression was most strongly promoted by IL-4 and the additive or synergistic effects are not recognized between these cytokines. On the other hand, Secretion of SC in medium after the culture was nothing or very little in I groups. Thus, it was indicated that PRL plays an important role in SC secretion from mammary cells. (author abst.) |
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