Effect of Nitric Oxide on Bovine Adipocyte Differentiation
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Accession number;04A0022339
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| Title;Effect of Nitric Oxide on Bovine Adipocyte Differentiation |
| Author;
YANO HIDEO
(Kyoto Univ., Graduate School of Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.138-142(2003)
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| Figure&Table&Reference;FIG.4, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Nitric oxide(NO) has been suggested to affect lipid metabolism. The suppression of NO production increased body fat. We investigated gene expression of endothelial nitric oxide synthase(eNOS,) and inducible nitric oxide synthase(iNOS) in bovine muscular tissue, adipose tissue and stromal vascular cells derived from bovine adipose tissue. We also examined the effects of a NO donor(2,2'-(hydroxynitrosohydrazono)bis-ethanamine, NOC18) and a NOS inhibitor(N.OMEGA.-nitro-L-arginine methylester hydrochloride, L-NAME) on bovine adipocyte differentiation using the stromal vascular cells. The NO donor suppressed adipocyte differentiation, which suggested that exogenous NO suppressed bovine adipocyte differentiation. The treatment with L-NAME stimulated adipocyte differentiation and L-arginine suppressed the stimulative effect of L-NAME. These results indicated that endogenous NO also suppressed bovine adipocyte differentiation. Both NOS genes were expressed in bovine muscular tissue and adipose tissue. Gene expression of iNOS was also found in each stromal vascular cell culture and eNOS mRNA was detected in a culture. Because adipocytes are located along with vessels in bovine muscle, intramuscular adipocyte differentiation is likely to be regulated by eNOS producing NO in the endothelial cells of vessel. (author abst.) |
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