Mechanism of Fat Marbling in Muscle by Leptin and Vascular Endothelial Growth Factor in Beef Cattle
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Accession number;04A0022341
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| Title;Mechanism of Fat Marbling in Muscle by Leptin and Vascular Endothelial Growth Factor in Beef Cattle |
| Author;
OBARA YOSHIAKI
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.150-154(2003)
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| Figure&Table&Reference;TBL.3, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The aim of this study was to investigate the mechanism of leptin expression in bovine adipocyte using boine intramuscular preadipocyte(BIP) cell line established by Aso et al(8). 1) Leptin expression was observed in growing BIP cells before differentiation and was considered to be corresponded with cell proliferation. 2) After the incubation with differentiation medium containing acetic acid, octanoic acid, insulin and dexamethasone, leptin mRNA expression was detected at day-6 and day-8, whereas leptin expression was not observed from day-0 to day-4. 3) Leptin concentrations decreased by the culture with differentiation medium, and leptin was not detected in cytosol fraction at day-4. It considered to be caused, at least partly, by dexamethasone, a differentiation factor used in this study. Leptin concentrations increased at day-6 and day-8 compared with that at day-4. 4) Acetic acid, a major energy source for ruminant animals, increased leptin expression compared with control and all other treatments. In conclusion, leptin mRNA expression in BIP cells increased by differentiation to mature adipocyte. These results concur with previous reports on non-ruminant animals. Leptin expression was also observed in premature preadipocyte. However, control of leptin expression in preadipocyte may differ from that of mature adipocyte. Leptin expressed in preadipocyte is considered to be correlated with that vascular endothelial growth in the adipose tissue. (author abst.) |
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