Association of Intramuscular Fat with Candidate Genes in Duroc Pigs

Accession number;04A0022342
Title;Association of Intramuscular Fat with Candidate Genes in Duroc Pigs
Author; SUZUKI KEIICHI (Tohoku Univ., Graduate School of Agricultural Sci., JPN) UEMOTO YOSHINOBU (Tohoku Univ., Graduate School of Agricultural Sci., JPN) SHIBATA TOMOYA (Miyagi Prefectural Livestock Experiment Station, JPN) KADOWAKI HIROSHI (Miyagi Prefectural Livestock Experiment Station, JPN) NISHIDA AKIRA (Tohoku Univ., Graduate School of Agricultural Sci., JPN)
Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
Journal Code:X0296A
ISSN:
VOL.21;NO.;PAGE.155-160(2003)
Figure&Table&Reference;TBL.5, REF.12
Pub. Country;Japan
Language;Japanese
Abstract;In order to conduct selection for intramuscular fat(IMF) efficiently, the relation between IMF and H-FABP(Heart Fatty Acid Binding Protein) gene which was the candidate gene was examined. First of all, the change in each RFLP frequency of the H-FABP gene according to the selection generation was examined by using the Duroc breed to which the intramuscular fat was improved as a selected trait. Next, it was examined whether there was a difference of IMF content between H-FABP gene polymorphisms. Moreover, the effect of the genotype was presumed, and how RFLP of the H-FABP gene influenced IMF content was examined. Msp I polymorphism and Hae III polymorphism frequency of H-FABP genes showed a similar change along with the selection. The breeding value of AA was significantly higher than that of Aa and aa in Msp I polymorphism. The breeding values of the Hh and hh were significantly higher than that of HH in Hinf I polymorphism and the breeding value of DD was significantly higher than that of Dd and dd in Hae III polymorphism. The breeding values of AA/DD/Hh and AA/DD/hh were the highest in the haplotype, and AA/dd/HH was lowest. The breeding values of Msp I polymorphism, Hae III polymorphism, and Hinf I polymorphism were assumed to be a independent variates, and multiple regression analysis of which the dependent variate was the entire breeding value was done. As a result, the partial regression coefficient of Msp I polymorphism and Hae III polymorphism became statistically significant. And, the contribution rate was 0.389 when Msp I polymorphism and Hae III polymorphism were assumed to be a independent variate, and it can be to explain about 40 percent of the total variances of IMF breeding value by the difference of these two DNA polymorphisms. (author abst.)