Purification of an Antilisterial Bacteriocin Produced by Brochothrix thermosphacta No.3 and Characterization of the Genetic Locus Responsible for Its Biosynthesis
|
Accession number;04A0022353
|
| Title;Purification of an Antilisterial Bacteriocin Produced by Brochothrix thermosphacta No.3 and Characterization of the Genetic Locus Responsible for Its Biosynthesis |
| Author;
KATSUMATA RYOICHI
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
ANDO TASUKE
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
YONEYAMA HIROSHI
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
|
Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
|
Journal Code:X0296A
|
ISSN:
|
|
VOL.21;NO.;PAGE.220-224(2003)
|
| Figure&Table&Reference;FIG.2, TBL.2, REF.10 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;A bacteriocin produced by Brochothrix thermosphacta No.3, which exhibited an antibacterial activity against Listeria monocytogenes in refrigerated packed meat, was purified from culture supernatant through ammonium sulfate precipitation, anion exchange chromatography, and gel filtration chromatography. SDS-PAGE analyses of the preparation obtained showed a single protein band with a molecular weight of about 10kDa and antilisterial activity, indicating that the bacteriocin consists of this protein monomer. Since strain MY1 derived from strain No.3 by acriflavine treatment lost productivity of and immunity to the bacteriocin, the genes associated with bacteriocin production were suggested to be encoded by a plasmid. While strain No.3 contained a large plasmid(pBTL, 78kb) and a small plasmid(pBTS, 3.1kb), pBTL disappeared in strain MY1. When plasmid pBV11, in which pBTS was coupled on to an Escherichia coli vector pVA981 containing a tetracycline resistance gene from a Gram-positive bacterium, was transformed into strain No.3 by selecting for tetracycline resistance, the resulting transformant lost pBTS due to action of plasmid incompatibility. Subsequent curing of pBV11 from this transformant by screening for sensitivity to tetracycline generated the strain MY2 carrying only pBTL. Strain MY2 retained bacteriocin productivity and immunity function. It was thus confirmed that the genes related to bacteriocin production in B. thermosphacta No.3 are encoded by pBTL. (author abst.) |
|
|
|
Related Articles;
|
|
