Science Links Japan | Priminary Study of Detecting System for Contamination due to Bacteria and Fungi in Meats and Dairy Products

Priminary Study of Detecting System for Contamination due to Bacteria and Fungi in Meats and Dairy Products

Accession number;04A0022354

Title;Priminary Study of Detecting System for Contamination due to Bacteria and Fungi in Meats and Dairy Products

Author; IKEDA TERUO (Azabu Univ., Sch. of Vet. Med.) FUNABA MASAYUKI (Azabu Univ., Sch. of Vet. Med.)

Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho

Journal Code:X0296A

ISSN:

VOL.21;NO.;PAGE.225-230(2003)

Figure&Table&Reference;FIG.5, REF.22

Pub. Country;Japan

Language;Japanese

Abstract;Dairy products and meats may contain a large amount of endotoxin and .BETA.-glucan because it could be contaminated with microorganisms during processing to products. For the reason of safety and human welfare, the endotoxin and .BETA.-glucan levels in foods should be limited for preventing the diseases to take a great amount of endotoxin and .BETA.-glucan. To assess the contamination of meats and dairy products, we investigated the detectable usefulness of endotoxin and .BETA.-glucan specific limulus test using chromogenic substrate for screening products contaminated with bacterial and fungal constitutions as previous report. Among commercial milk, sterilized milk, heated at 130.DEG.C. for 2 second(UHT method), had an especially high value of endotoxin(mean: 220.72EU/ml), but a few rots of milks showed a low level of endotoxin(mean: 1.31EU/ml). On the other hand, pasteurized milk, heated at 62-65.DEG.C. for 30min(LTLT method), contained low concentration of endotoxin(0.27EU/ml) as well as previously reported. There was a significant difference between LTLT and UHT method in heating process. In meats, pork and chicken contained high level of endotoxin(mean 19952.99 and 5943.08pg/ml) in comparison to them in beef(219.40pg/ml). In both of milk and meats, .BETA.-glucan levels were normal(<102pg/ml). While gastrointestinal tract has a major functions of digestion and absorption of nutrients, their mucus act as a defense barrier against microorganisms and toxins invasion which derived from contamination of food and drinking nutrients. Collapse of gastrointestinal barrio often occurs in many clinical syndromes such as hemorige shock, surgically invasion, resulting acceleration of translocation with bacteria or/and endotoxin into tissue or/and blood stream from gut.... (author abst.)

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