Protective Roles of Nutritional Factors from Meat in the Development of Central Nervous System
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Accession number;04A0022356
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| Title;Protective Roles of Nutritional Factors from Meat in the Development of Central Nervous System |
| Author;
YANAKA NORIYUKI
(Hiroshima Univ., Graduate School of Biosphere Sci., JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.236-238(2003)
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| Figure&Table&Reference;FIG.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The aim of this study is to identify the genes modified by beef oil feeding for 4 weeks using the differential display method. In the present study, we found that expression of two novel genes, TAK21 and S6A was regulated by beef oil diet, suggesting that the effect of beef oil feeding on the brain work might be partially mediated by the change of expression level of the two novel genes. Nine male CD-1(ICR, 5 week old, Charles River Japan Inc., Hino, Japan) were kept in a metal cage at a controlled temperature in a 12 hour light: dark cycle. They had free access to diet and deionized water. After being fed commercial stock diet(MF, Oriental Yeast) for 1 week, the mice were divided into four groups of 9 mice. The four groups fed the experimental diets containing 5% corn oil(LC), 20% corn oil(HC), 5% beef oil(LB), and 20% beef oil(HB), respectively. The feeding experimental period was 28 days. Body weighing and preparation of both diets were preformed every day. The brains from the mice sacrificed by cervical dislocation were immediately removed and frozen in liquid nitrogen. Total RNA was isolated from the brains using ISOGEN according to the manufacture's protocol. The total RNAs were used for the differential display method and for Northern blot analyses. Briefly, total RNA(5.MU.g) from brains of LC and HB groups was converted to cDNA with first-strand synthesis kit(Amersham Pharmacia Biosciences). Subsequently, each pool of cDNA was amplified by PCR with 200 different arbitrary primers. After separation by 5% polyacrylamide gel electrophoresis, cDNA bands that appeared to be up-regulated or down-regulated were recovered and reamplified with the corresponding primer. Reamplifed cDNA fragments were cloned into pGEM-T vector(Promega). The cDNA inserts from true positive clones were sequenced by dideoxy chain termination methods using an Applied Biosystems Model 373A automated sequencer.... (author abst.) |
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