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Accession number;04A0022357
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| Title;Study of a New Bioactive Substance, Spinorphin, Purified from Bovine Spinal Cord |
| Author;
HAZATO TADAHIKO
(Tokyo Metrop. Inst. of Med. Sci.)
YAMAMOTO YUKIO
(Tokyo Metrop. Inst. of Med. Sci.)
SHIMAMURA MARIKO
(Tokyo Metrop. Inst. of Med. Sci.)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.239-243(2003)
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| Figure&Table&Reference;FIG.3, REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Spinorphin has been isolated from the bovine spinal cord as an endogenous inhibitor of enkephalin-degrading enzymes. Structural analysis revealed the factor to comprises Leu-Val-Val-Tyr-Pro-Trp-Thr(LVVYPWT: Mw=876). Recently, by measuring flexor responses induced by the intraplanter injection of bradykinin and spinorphin, the analgesia were evaluated. bradykinin(BK)-induced nociceptive responses were blocked by spinorphin. Thus, it is suggested that the blockade is induced by the spinorphin binding to receptors associated with analgesia. The rat spinal cords were incubated with [3H]spinorphin and increasing concentrations of unlabelled spinorphin. Saturation binding studies using increasing concentrations of [3H]spinorphin indicated that spinal cord membranes contained high affinity spinorphin binding sites with Kd of 16nM for [3H]spinorphin and a Bmax of 3.9pmol/mg. Spinorphin plays important roles in analgesia, possibly by binding this receptors. (author abst.) |
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