Applied Study of Opioid Peptide from Domestic Animals to Anti-inflammatory Drug(3)-Inhibitory mechanism of Hemorphin 6 on antigen-induced neurogenic inflammation in sensitized guinea pig airways-
|
Accession number;04A0022358
|
| Title;Applied Study of Opioid Peptide from Domestic Animals to Anti-inflammatory Drug(3)-Inhibitory mechanism of Hemorphin 6 on antigen-induced neurogenic inflammation in sensitized guinea pig airways- |
| Author;
YOSHIHARA SHIGEMI
(Dokkyo Univ. Sch. of Med.)
YAMADA YUMI
(Dokkyo Univ. Sch. of Med.)
WATANABE SHIGEAKI
(Ito Ham Foods Inc., JPN)
ABE TOSHIO
(Dokkyo Univ. Sch. of Med.)
ARISAKA OSAMU
(Dokkyo Univ. Sch. of Med.)
|
Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
|
Journal Code:X0296A
|
ISSN:
|
|
VOL.21;NO.;PAGE.244-247(2003)
|
| Figure&Table&Reference;FIG.3, REF.8 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We investigated the potential of Hemorphin-6 to reduce antigen-induced neurogenic plasma extravasation in guinea pig airways. We measured the antigen-induced plasma extravasation in guinea pig trachea, as an index of neurogenic inflammation. The increase in plasma extravasation induced by antigen challenge(OVA: 5%, 2min, inhalation) was significantly reduced by pretreatment with Hemorphin-6(1mg/kg, i.v.). Hemorphin-6(1mg/kg, i.v.) inhibited by 50% in the trachea plasma extravasation induced by OVA challenge. Furthermore, we examined affinity of Hemorphin-6 to opioid receptors(.DELTA.,.KAPPA.,.MU.) using radioligand binding method in vitro. Binding inhibition to opioid .DELTA.,.KAPPA. and .MU. receptors of Hemorphin-6 was 12%, 8% and 47% respectively. Opioid .MU. receptor antagonist, endomorphin-1 inhibited by 99% in the binding to opioid .MU. receptor. These findings suggest that Hemorphin-6 reduces antigen-induced plasma extravasation by inhibiting the release of substance P from primary sensory nerves(C-fiber) via prejunctional .MU.-opioid receptors in sensitized guinea pig airways. (author abst.) |
|
|
|
Related Articles;
|
|
