Search of Novel Biologically Active Components in Liver Hydrolysates
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Accession number;04A0022359
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| Title;Search of Novel Biologically Active Components in Liver Hydrolysates |
| Author;
MUGURUMA MICHIO
(Miyazaki Univ., Faculty of Agriculture, JPN)
NISHI TOSHIO
(Miyazaki Univ., Faculty of Agriculture, JPN)
OKUNO MAYO
(Miyazaki Univ., Faculty of Agriculture, JPN)
YAMASHITA MAYUKO
(Miyazaki Univ., Faculty of Agriculture, JPN)
KAWAHARA SATOSHI
(Miyazaki Univ., Faculty of Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.21;NO.;PAGE.248-255(2003)
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| Figure&Table&Reference;FIG.5, REF.17 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Liver hydrolyzed powder(Liver-Hi) contains the various functional materials, which has bioregulation. Therefore, it is considered that the functional materials are existed in the liver-Hi. The purpose of this study was to search their functional materials, especially angiotensin I-converting enzyme(ACE) inhibitory peptides and antioxidant activity. First of all, inhibitory activities against ACE of liver-Hi and their enzymatic hydrolysates were investigated. Liver-Hi was digested by pepsin, trypsin and .ALPHA.-chymotrypsin. After digestion, hydrolysates showed ACE inhibitory activities, and the tryptic hydrolysates showed the strongest activity. The liver-Hi ethanol extract was applied to reverse-phase high performance liquid chromatography to separate an active peptide. In the case of A-18 fraction, the molar concentration of liver-Hi peptides required to inhibit 50% of the activity increased markedly(0.268.MU.g/ml). Secondary, the effects of liver-Hi and their enzymatic hydrolysates on antioxidant activities were studied. The DPPH radical scavenging method and the hydroxyl radical scavenging method were used for detecting. The liver-Hi had strong antioxidant activity. The molar concentration of liver-Hi peptides required to inhibit 50% of the activity of DPPH radical scavenging and hydroxyl radical scavenging were 157.MU.g/ml and 135.MU.g/ml, respectively. These results suggested that the porcine liver-Hi was able to show ACE inhibitory activity and antioxidant activity, and that might be a useful source of physiologically functional factors. (author abst.) |
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