Preventive Effect of Juzen-taiho-to on Endometrial Carcinogenesis in Mice Is Based on Shimotsu-to Constituent
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Accession number;04A0108785
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| Title;Preventive Effect of Juzen-taiho-to on Endometrial Carcinogenesis in Mice Is Based on Shimotsu-to Constituent |
| Author;
TAGAMI K
(Gifu Univ. School Of Medicine, Gifu, Jpn)
NIWA K
(Gifu Univ. School Of Medicine, Gifu, Jpn)
LIAN Z
(Gifu Univ. School Of Medicine, Gifu, Jpn)
GAO J
(Gifu Univ. School Of Medicine, Gifu, Jpn)
MORI H
(Gifu Univ. School Of Medicine, Gifu, Jpn)
TAMAYA T
(Gifu Univ. School Of Medicine, Gifu, Jpn)
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Journal Title;Biol Pharm Bull
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Journal Code:S0989A
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ISSN:0918-6158
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VOL.27;NO.2;PAGE.156-161(2004)
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| Figure&Table&Reference;FIG.3, TBL.5, REF.37 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Juzen-taiho-to, a Kampo formula, originally consists of a mixture of Shimotsu-to and Shikunshi-to formulas together with two other crude ingredients. Juzen-taiho-to is reported to have a preventive effect on endometrial carcinogenesis in mice. Shimotsu-to exerts an inhibitory effect on estrogen-induced expression of c-fos, interleukin (IL)-1.ALPHA. and tumor necrosis factor (TNF)-.ALPHA. in uteri of ovarectomized mice. In the present study, short- and long-term experiments were designed to determine the effects of Juzen-taiho-to and Shimotsu-to on the estrogen-related endometrial carcinogenesis in mouse uteri, associated with the expression of cyclooxygenase (COX)-1 and -2. In the short-term experiment, exposure to Juzen-taiho-to or Shimotsu-to significantly reduced estradiol-17.BETA. (E2)-stimulated expressions of COX-2 mRNA (p<0.05) as well as the protein. However, no effects on the expression of COX-1 were observed. Shikunshi-to did not affect COX expression. In the long-term experiment, 90 female ICR mice were given N-methyl-N-nitrosourea (MNU) into their uterine corpora. The animals were divided into four groups as follows: group 1, a diet containing 0.07% Shimotsu-to and 5 ppm E2; group 2, a diet containing 5 ppm E2; group 3, a diet containing 0.07% Shimotsu-to; group 4 served as a control. Exposure of Shimotsu-to reduced the incidence of MNU- and E2-induced endometrial adenocarcinoma and atypical hyperplasia at the termination of the experiment (30 weeks). The above findings and our previous reports suggest that Shimotsu-to is responsible for the preventive effects of Juzen-taiho-to on estrogen-related endometrial carcinogenesis in mice, through the inhibition of estrogen-related COX-2 as well as c-fos, IL-1.ALPHA. and TNF-.ALPHA. expressions. (author abst.) |
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