Regulatory mechanisms of lipogenesis in cultured hamster sebocytes.
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Accession number;04A0230057
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| Title;Regulatory mechanisms of lipogenesis in cultured hamster sebocytes. |
| Author;
SATO TAKASHI
(Tokyo Coll. of Pharm., Fac. of Pharm. Sci.)
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Journal Title;Fragr J
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Journal Code:G0987B
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ISSN:0288-9803
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VOL.32;NO.3;PAGE.14-18(2004)
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| Figure&Table&Reference;FIG.5, TBL.1, REF.22 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Sebaceous glands are important skin appendages and sebum excretion is considered to be associated with functionally maintaining the cutaneous surface as a biological barrier. However, the regulatory mechanisms of lipogenesis in sebaceous glands are not fully understood. Therefore, we investigated the effect of epidermal growth factor (EGF), transforming growth factor-.ALPHA. (TGF-.ALPHA.), basic fibroblast growth factor (bFGF) and keratinocyte growth factor (KGF), all-trans retinoic acid (RA), l.ALPHA.,25-dihydroxyvitamin D3 (VD3) and 5.ALPHA.-dihydrotestosterone (5.ALPHA.DHT) on lipogenesis in cultured hamster sebocytes. The sebocytes are found to spontaneously differentiate to accumulate intracellular lipid droplets, which consisted mainly of triacylglycerols (TGs) (71.8%) and, as minor components, cholesterol, wax esters and free fatty acids. The formation of lipid droplets and the synthesis of TGs were diminished by RA, EGF, TGF-.ALPHA., bFGF, and slightly by KGF and VD3, whereas 5 .ALPHA. DHT augmented the formation of intracellular lipid droplets along with an increase in TGs levels in hamster sebocytes. In addition, EGF, TGF-.ALPHA. and bFGF, but not KGF, augmented the proliferation of hamster sebocytes. These results suggest that hamster sebocytes are useful for the elucidation of sebaceous function at the cellular level. Furthermore, this is the first evidence that EGF, TGF-.ALPHA., bFGF, KGF and VD3 may act as suppressors in the regulation of lipogenesis and have mitogenic activity in sebaceous glands. (author abst.) |
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