Assessment of Conjunctival Impression Cytology Method (CIC) of .BETA.-Carotene and Canthaxanthin Fed Vitamin A Defficient Rats
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Accession number;04A0049344
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| Title;Assessment of Conjunctival Impression Cytology Method (CIC) of .BETA.-Carotene and Canthaxanthin Fed Vitamin A Defficient Rats |
| Author;
()
INOUE HIROAKI
(Nihon Univ., Coll. Bioresource Sci., JPN)
TATEISHI AKIRA
(Nihon Univ., Coll. Bioresource Sci., JPN)
HASHIMOTO HIDEKI
(Osaka City Univ., Graduate School of Sci., JPN)
TOMITA YOSHIFUMI
(Kyushu Kyoritsu Univ., Faculty of Engineering, JPN)
MURAKOSHI MICHIAKI
(Lion Corp., JPN)
()
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Journal Title;Journal for the Integrated Study of Dietary Habits
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Journal Code:L3330A
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ISSN:1346-9770
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VOL.14;NO.3;PAGE.194-199(2003)
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| Figure&Table&Reference;FIG.3, TBL.3, REF.12 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In this research the, .BETA.-carotene, canthaxanthin, and those with added cholic acid were fed to vitamin A deficient rats to investigate the retinal conversion by using conjunctiva trace cell collection method. In order to check the generation and differentiation of normal cell for .BETA.-carotene, canthaxanthin, and those with added cholic acid, conjunctiva trace cell were collected by filter film from 7 blocks 42 samples. After dyeing, microscopic examination was carried out. The results were categorized into 6 levels by the shape of the damage of the generation and differentiation of cells. The cells are further categorized into normal cells, borderline cells, and abnormal cells, to be finally investigated for the correlation between the amount of normal cells and the formation of retinal. The result shows that the.BETA.-carotene groups, especially the .BETA.-carotene group with added cholic acid, have remarkably high number of normal cells. There were also much in retinyl acetate block. There were relatively few in canthaxanthin block, and the canthaxanthin group with added cholic acid showed a slight decrease. The retinal formation for all the blocks showed proportional positive correlation. The result supports the fact that conjunctiva trace cell test is effective for examining the retinal formation induced by carotenoid. |
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