Simple Identification and Measurement of Vitamin B12 (cyanocobalamin) Analogue Contained in a Bamboo Shoot (Phyllostachys)
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Accession number;04A0049347
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| Title;Simple Identification and Measurement of Vitamin B12 (cyanocobalamin) Analogue Contained in a Bamboo Shoot (Phyllostachys) |
| Author;
TAMIYA KATSUYOSHI
(Himeji Inst. Technol., JPN)
NAKAI TOMONORI
(Himeji Inst. Technol., JPN)
YAMAUCHI DAISUKE
(Himeji Inst. Technol., JPN)
KUBOTA KO
(Himeji Inst. Technol., JPN)
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Journal Title;Journal for the Integrated Study of Dietary Habits
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Journal Code:L3330A
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ISSN:1346-9770
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VOL.14;NO.3;PAGE.215-220(2003)
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| Figure&Table&Reference;FIG.3, TBL.2, REF.10 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Using bamboo embryo plant which is thought to contain vitamin12 analogue, the vitamin 12 analogue was detected by simple identification method. 0.1% KCN was added to the extraction buffer solution, and by using anion exchange column and reversed phase column, vitamin 12 was separated from bamboo embryo plant. During the fraction equal to the dissolution of cyanocobalamine used as the index, absorption maximum wavelength was observed at 361nm and 550nm. The absorption spectrum was especially strong at 361nm, and the possibility of cyanocobalamine included in this fraction was suggested. By mass spectrometric analysis revealed the existence of signal (1346.38) which is very close to cyanocobalamine (1331.42). This showed the possibility of detecting cyanocobalamine by the method introduced in the paper. In addition, crude extract of embryo plants of rice plant and asparagus that are thought not to contain vitamin 12 were separated by the same method and were examined for the absorption spectrum in the range from 200nm to 600nm. Absorption maximum wavelength characteristic to cyanocobalamine were not detected for both plants, and were considered that these plants do not contain vitamin 12. The method seems to be an effective method to detect vitamin 12 analogues. |
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